| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Luddecke, Megan B (11)
|
MAWGY |
34.01 |
33.29 |
-0.72 |
| 2 |
Murphy, Kristen Y (12)
|
MARKRY |
33.37 |
33.62 |
0.25 |
| 3 |
Schofield, Abigail L (12)
|
MARKRY |
35.08 |
34.47 |
-0.61 |
| 4 |
Wicentowski, Emma S (12)
|
MARKRY |
35.85 |
34.80 |
-1.05 |
| 5 |
Stewart, Ava (12)
|
MABCY |
37.49 |
34.82 |
-2.67 |
| 6 |
Terry, Allison (12)
|
MABCY |
|
35.24 |
- |
| 7 |
Chidester, Cadence (11)
|
MABCY |
|
35.42 |
- |
| 8 |
Savadove, Kou M (11)
|
MAWGY |
36.65 |
35.46 |
-1.19 |
| 9 |
Ohm, Kamryn J (12)
|
MARKRY |
36.75 |
35.60 |
-1.15 |
| 10 |
Youngblood, Kailey M (12)
|
MALYM |
37.02 |
35.83 |
-1.19 |
| 11 |
Dougherty, Renee T (12)
|
MARKRY |
38.30 |
36.37 |
-1.93 |
| 12 |
Murphy, Chloe (11)
|
MALYM |
36.05 |
37.00 |
0.95 |
| 13 |
Smallwood, Charlotte R (11)
|
MARKRY |
37.38 |
37.02 |
-0.36 |
| 14 |
Mendel, Sadie M (12)
|
MAWGY |
37.54 |
37.19 |
-0.35 |
| 15 |
Weedon-Miller, Anika L (11)
|
MALYM |
37.07 |
37.78 |
0.71 |
| 16 |
Omlor, Olivia L (12)
|
MARKRY |
38.86 |
38.39 |
-0.47 |
| 17 |
Kelly, Erin E (12)
|
MALYM |
39.67 |
39.00 |
-0.67 |
| 18 |
Parnati, Ridhima (11)
|
MAUMLY |
38.61 |
39.08 |
0.47 |
| 19 |
Fischbach, Emily K (12)
|
MALYM |
39.57 |
40.44 |
0.87 |
| 20 |
Knouft, Keira A (11)
|
MAUMLY |
41.61 |
40.46 |
-1.15 |
| 21 |
Gupta, Hannah (12)
|
MAUMLY |
42.65 |
40.66 |
-1.99 |
| 22 |
Look, Sabrina M (12)
|
MAUMLY |
42.66 |
42.03 |
-0.63 |
| 23 |
Spears, Hannah (12)
|
MARKRY |
40.66 |
42.24 |
1.58 |
| 24 |
Karlson, Katherine E (11)
|
MAWGY |
42.73 |
42.44 |
-0.29 |
| 25 |
Harris, Joanna S (11)
|
MAUMLY |
44.86 |
42.74 |
-2.12 |
| 26 |
Weissenburger, Emily G (11)
|
MARKRY |
44.60 |
46.27 |
1.67 |
| 27 |
Wang, Emily E (11)
|
MAUMLY |
|
49.50 |
- |
| 28 |
Poole, Katerina E (12)
|
MALYM |
56.84 |
53.79 |
-3.05 |
| 29 |
Whitfield, Leilani (11)
|
MABCY |
1:08.22 |
58.28 |
-9.94 |
|
Holland, Genevieve A (12)
|
MAWGY |
36.87 |
DQ |
- |